Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody

The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl)porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques.The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS.The subtype of MAb 1F2 is IgG2a, which has a relative molecular weight of 156,678.8 Da.No significant change in the intensity of absorption peaks in UV and CD spectra was observed over a pH range between 6 and 12.The high stability of the abzyme and the tight binding between Fe porphyrin and antibody were also demonstrated.Vmax, Km, κcat, κcat/Km for abzyme are 5.18 × 10 -8 Ms, 1.50 × 10M , 0.518 s, 3.45 × 10 Ms, respectively.The data obtained indicate that catalytic antibody has high catalytic activity.The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stable from 10 °C to 60 °C. Int.J.Mol.Sci.2007, 8 446